Author: Jiménez-Clavero, Miguel Á
Title: Animal viral diseases and global change: bluetongue and West Nile fever as paradigms Document date: 2012_6_13
ID: wvm2ua95_42
Snippet: The virus is propagated in the reservoir hosts, resulting in a viremic phase that usually lasts no more than 5-7 days (Komar et al., 2003) . The duration and level of viremia depends on the species infected (Komar et al., 2003) . The detection of the virus or its genetic material in serum or cerebrospinal fluid in a laboratory test is a proof of diagnostic value (De Filette et al., 2012) . The virus is evidenced by virological (virus isolation) o.....
Document: The virus is propagated in the reservoir hosts, resulting in a viremic phase that usually lasts no more than 5-7 days (Komar et al., 2003) . The duration and level of viremia depends on the species infected (Komar et al., 2003) . The detection of the virus or its genetic material in serum or cerebrospinal fluid in a laboratory test is a proof of diagnostic value (De Filette et al., 2012) . The virus is evidenced by virological (virus isolation) or molecular (RT-PCR-conventional and real-time, NASBA) techniques. In epidemiological surveillance it is useful to detect the presence of WNV in mosquitoes, for which they are homogenized and analyzed using the same methods mentioned above (Trevejo and Eidson, 2008) . Specific antibodies against the virus are detectable in blood few days after infection (Komar et al., 2003; De Filette et al., 2012) . Antibody detection is performed by serological tests (enzyme immunoassay or ELISA, hemagglutination inhibition or HIT) which can be confirmed by more specific serological techniques (virus-neutralization test; Sotelo et al., 2011c) . Serological diagnosis of acute infection should be done by detection of IgM antibodies in serum and/or cerebrospinal fluid using an immunocapture ELISA together with the detection of an increase in antibody titer in paired sera taken one in the acute phase and the other, at least 2 weeks later (Beaty et al., 1989) .
Search related documents:
Co phrase search for related documents- acute infection and antibody titer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
- acute infection and antibody titer increase: 1
- acute infection and cerebrospinal fluid: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60
- acute infection and cerebrospinal fluid serum: 1, 2, 3, 4, 5, 6
- acute infection and diagnostic value: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- acute infection and ELISA enzyme immunoassay: 1
- acute infection and enzyme immunoassay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- acute infection and epidemiological surveillance: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19
- acute infection and genetic material: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- acute infection and hemagglutination inhibition: 1, 2, 3
- acute infection and IgM antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44
- acute infection and IgM antibody detection: 1, 2, 3, 4, 5
- acute infection and laboratory test: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28
- acute infection and level duration: 1, 2, 3, 4, 5, 6, 7
- acute infection and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
- acute infection and reservoir host: 1, 2, 3
- acute infection and serological diagnosis: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- acute infection and serological test: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- acute infection and serum IgM antibody detection: 1
Co phrase search for related documents, hyperlinks ordered by date