Author: Bederka, Lydia H.; Bonhomme, Cyrille J.; Ling, Emily L.; Buchmeier, Michael J.
Title: Arenavirus Stable Signal Peptide Is the Keystone Subunit for Glycoprotein Complex Organization Document date: 2014_10_28
ID: wbh06gzb_29
Snippet: Cell culture and virus stocks. BHK-21, DBT, HEK 293T, and Vero E6 cell lines were maintained at 37°C, 5% CO 2 , with 1ϫ Dulbecco's modified Eagle's medium (DMEM), 10% fetal bovine serum, penicillinstreptomycin, and L-glutamine. The LCMV Armstrong 4 (Arm-4) strain was used in this report (51, 52) . Viral stocks were propagated in BHK-21 cells, and viral titers were determined via plaque assay using Vero E6 cells. Transient transfections were per.....
Document: Cell culture and virus stocks. BHK-21, DBT, HEK 293T, and Vero E6 cell lines were maintained at 37°C, 5% CO 2 , with 1ϫ Dulbecco's modified Eagle's medium (DMEM), 10% fetal bovine serum, penicillinstreptomycin, and L-glutamine. The LCMV Armstrong 4 (Arm-4) strain was used in this report (51, 52) . Viral stocks were propagated in BHK-21 cells, and viral titers were determined via plaque assay using Vero E6 cells. Transient transfections were performed using Lipofectamine 2000 as per the manufacturer's protocol (Life Technologies).
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