Author: Zirkel, Florian; Kurth, Andreas; Quan, Phenix-Lan; Briese, Thomas; Ellerbrok, Heinz; Pauli, Georg; Leendertz, Fabian H.; Lipkin, W. Ian; Ziebuhr, John; Drosten, Christian; Junglen, Sandra
Title: An Insect Nidovirus Emerging from a Primary Tropical Rainforest Document date: 2011_6_14
ID: ulwo6i38_9
Snippet: Full genome sequencing of isolate CAVV/C79/CI/2004 was achieved by a combination of adaptor-based random RT-PCR and ultradeep sequencing. The entire CAVV genome comprised 20,108 nt, excluding the 3= poly(A) tail (GenBank accession number HM746600), a size intermediate between that of arteriviruses (13 to 16 kb) and CoV or RoV (26 to 32 kb) (17) . The genome contained seven major ORFs, as well as untranslated regions of 362 and 570 nt at the 5= an.....
Document: Full genome sequencing of isolate CAVV/C79/CI/2004 was achieved by a combination of adaptor-based random RT-PCR and ultradeep sequencing. The entire CAVV genome comprised 20,108 nt, excluding the 3= poly(A) tail (GenBank accession number HM746600), a size intermediate between that of arteriviruses (13 to 16 kb) and CoV or RoV (26 to 32 kb) (17) . The genome contained seven major ORFs, as well as untranslated regions of 362 and 570 nt at the 5= and 3= ends, respectively ( Table 2 ; Fig. 4A ).
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