Author: Zirkel, Florian; Kurth, Andreas; Quan, Phenix-Lan; Briese, Thomas; Ellerbrok, Heinz; Pauli, Georg; Leendertz, Fabian H.; Lipkin, W. Ian; Ziebuhr, John; Drosten, Christian; Junglen, Sandra
Title: An Insect Nidovirus Emerging from a Primary Tropical Rainforest Document date: 2011_6_14
ID: ulwo6i38_26
Snippet: Electron microscopy. For electron microscopy, viral particles were purified through a cushion of 36% sucrose and the pellet was suspended in PBS (15, 16) . Viral particles were fixed with 2% paraformaldehyde and analyzed by transmission electron microscopy after negative staining with 1% uranyl acetate (62, 63) . For ultrathin sections, infected cells were fixed with 2.5% glutaraldehyde, enclosed in low-melting-point agar, embedded in resin, and .....
Document: Electron microscopy. For electron microscopy, viral particles were purified through a cushion of 36% sucrose and the pellet was suspended in PBS (15, 16) . Viral particles were fixed with 2% paraformaldehyde and analyzed by transmission electron microscopy after negative staining with 1% uranyl acetate (62, 63) . For ultrathin sections, infected cells were fixed with 2.5% glutaraldehyde, enclosed in low-melting-point agar, embedded in resin, and evaluated by transmission electron microscopy after ultrathin sectioning.
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