Author: Cho, Yong-il; Yoon, Kyoung-Jin
Title: An overview of calf diarrhea - infectious etiology, diagnosis, and intervention Document date: 2014_3_19
ID: uxghqdei_39
Snippet: There are several kinds of reporting dyes used for probe-based real-time PCR assays based on fluorescence energy wavelength. This facilitates multiplexing by combining different reporting dyes. Theoretically, multiplex real-time PCR can simultaneously detect up to four different targets in the same sample [18] . Nevertheless, there is a size limit for the PCR product (usually less than 200 bp) in order to maintain stable sensitivity [141] . The p.....
Document: There are several kinds of reporting dyes used for probe-based real-time PCR assays based on fluorescence energy wavelength. This facilitates multiplexing by combining different reporting dyes. Theoretically, multiplex real-time PCR can simultaneously detect up to four different targets in the same sample [18] . Nevertheless, there is a size limit for the PCR product (usually less than 200 bp) in order to maintain stable sensitivity [141] . The primers and probes should thus be carefully designed when a multiplex real-time PCR assay is performed. "Cross-talk" between different dyes due to close proximity in fluorescence energy wavelength is another factor to take into consideration when multiplexing.
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