Attractiveness of an Aggregation Pheromone Lure and Chicken Droppings to Adults and Larvae of Alphitobius diaperinus (Coleoptera: Tenebrionidae)

Singh, N.; Johnson, D. T.

Based on the total amount of all compounds present per microliter in CDV sample, the amount of CDV sample needed to get 10 g of total compounds was calculated (Table 1) . We decided to use 10 g based on preliminary bioassays results, where no signiÞcant differences were found among 10, 20, and 30 g CDV. This crude solution containing 10 g CDV compounds was then tested alone or in combination with the 20 g pheromone lure. Two grams of fresh CD was also tested singly or in combination with the 20 g pher-omone lure. Red rubber septa (11 mm, Wheaton, Millville, NJ) were cleaned in advance by soaking them in methylene chloride overnight. Each of these four treatments: 1) pheromone lure, 2) individual pheromone lure components, 3) CDV, and 4) pheromone lure ϩ CDV were applied separately to the rubber septa followed by 300 l methylene chloride, which was allowed to soak in and then the methylene chloride was allowed to evaporate for 2 h. Untreated control septa received 300 l methylene chloride.