Author: Henderson, Clark M.; Anderson, Christine B.; Howard, Michael T.
Title: Antisense-induced ribosomal frameshifting Document date: 2006_8_18
ID: xgwbl8em_29
Snippet: A second construct, p2luc-AZ1sl, was designed to contain only the 5 0 half of stem 1 of the antizyme pseudoknot downstream from the UCC UGA shift site ( Figure 1A ). 2 0 -O-Methyl antisense oligonucleotides were designed to anneal between 3 and 15 nt (SL1) or 3 and 22 nt (SL2) downstream from the UGA codon of the shift site. Frameshift efficiency induced by these two antisense oligonucleotides, 8 and 22% respectively, was somewhat lower than that.....
Document: A second construct, p2luc-AZ1sl, was designed to contain only the 5 0 half of stem 1 of the antizyme pseudoknot downstream from the UCC UGA shift site ( Figure 1A ). 2 0 -O-Methyl antisense oligonucleotides were designed to anneal between 3 and 15 nt (SL1) or 3 and 22 nt (SL2) downstream from the UGA codon of the shift site. Frameshift efficiency induced by these two antisense oligonucleotides, 8 and 22% respectively, was somewhat lower than that observed with PKm1 and PKm2 ( Figure 4C and D and Supplementary Table 4 ). In these cases frameshift efficiency was higher for the longer antisense oligonucleotide (SL2), suggesting that frameshift efficiency most probably correlates with stability of the duplex. As was seen with AZ1A, AZ1B and AZ1C, frameshifting efficiency stimulated by antisense oligonucleotides PKm1, PKm2, SL1 and SL2 was also strongly correlated with the concentration of exogenously added spermidine (Supplementary Table 5) .
Search related documents:
Co phrase search for related documents- antisense oligonucleotide and long antisense: 1
- antisense oligonucleotide and oligonucleotide long antisense: 1
- antisense oligonucleotide and shift site: 1, 2, 3, 4, 5
- antisense oligonucleotide and shift site UGA codon: 1, 2, 3
- antisense oligonucleotide and Supplementary table: 1, 2, 3
- antisense oligonucleotide and UGA codon: 1, 2, 3, 4
- duplex stability and frameshift efficiency: 1
- frameshift efficiency and shift site: 1, 2, 3, 4, 5, 6, 7, 8, 9
- frameshift efficiency and shift site UGA codon: 1, 2
- frameshift efficiency and Supplementary table: 1, 2, 3
- frameshift efficiency and UGA codon: 1, 2, 3, 4, 5, 6
- frameshifting efficiency and shift site: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
- frameshifting efficiency and shift site UGA codon: 1
- frameshifting efficiency and Supplementary table: 1
- frameshifting efficiency and UGA codon: 1, 2, 3, 4, 5
- long antisense and oligonucleotide long antisense: 1
- shift site and Supplementary table: 1, 2, 3
- shift site and UGA codon: 1, 2, 3, 4, 5, 6, 7, 8
- shift site UGA codon and Supplementary table: 1, 2
Co phrase search for related documents, hyperlinks ordered by date