Aurintricarboxylic acid increases yield of HSV-1 vectors

Pechan, Peter; Ardinger, Jeffery; Ketavarapu, Jyothi; Rubin, Hillard; Wadsworth, Samuel C; Scaria, Abraham

riments (n = 2) and are expressed as mean + SD. ATA, aurintricarboxylic acid; HSV, Herpes simplex virus. find that HSV-1 titer actually increases when ATA was at micromolar concentrations; however, a possible antiviral effect was observed when ATA was added into serum-free media. Because ATA, being a polycarboxylate, would bind by electrostatic interactions to any protein that contain positively charged residues given the myriad of possible interaction sites, it was considered as a nonspecific enzyme inhibitor. 20, 44 To establish an exact mechanism of action of ATA in HSV-1 yield increase, therefore, would simply remain unknown at this point. Our findings that ATA increases HSV-1 yield has important implications both for large-scale rHSV-1 production and rAAV vector production.