Selected article for: "PCR reaction and real time"
Author: Hassanzad, Maryam; Nadji, Seyed Alireza; Darougar, Sepideh; Tashayoie-Nejad, Sabereh; Boloursaz, Mohammad Reza; Mahdaviani, Seyed Alireza; Baghaie, Nooshin; Ghaffaripour, Hosseinali; Velayati, Ali Akbar
Title: Association of specific viral infections with childhood asthma exacerbations
  • Document date: 2018_9_27
    • ID: rqmjras4_9
    Snippet: Viral RNA and DNA were extracted together using the High Pure Viral Nucleic Acid Kit (Roche, Mannheim, Germany) from respiratory samples according to the manufacturer's recommendations, and 100 μl of nucleic acids was eluted. For RNA viruses, cDNA was synthesized using Superscript III reverse transcriptase (Invitrogen, Carlsbad, CA). The extracted genome elutes and cDNA were stored at −20°C for further studies with conventional and real-time .....
    Document: Viral RNA and DNA were extracted together using the High Pure Viral Nucleic Acid Kit (Roche, Mannheim, Germany) from respiratory samples according to the manufacturer's recommendations, and 100 μl of nucleic acids was eluted. For RNA viruses, cDNA was synthesized using Superscript III reverse transcriptase (Invitrogen, Carlsbad, CA). The extracted genome elutes and cDNA were stored at −20°C for further studies with conventional and real-time polymerase chain reaction (PCR).

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