Selected article for: "PCR assay and real time PCR assay"
Author: Yang Xu; Yi-rong Li; Qiang Zeng; Zhi-bing Lu; Yong-zhe Li; Wei Wu; Sheng-yong Dong; Gang Huang; Xing-huan Wang
Title: Clinical Characteristics of SARS-CoV-2 Pneumonia Compared to Controls in Chinese Han Population
  • Document date: 2020_3_10
    • ID: glq0lckz_8
    Snippet: A confirmed case of COVID-19 is defined as a positive result on high throughput sequencing or real-time reverse-transcriptase-polymerase-chain-reaction (RT-PCR) assay of pharyngeal swab specimens. Samples were collected for extracting SARS-CoV-2 RNA from patients suspected of having SARS-CoV-2 infection as described previously. 14 In brief, the pharyngeal swabs were placed into a collection tube with 150 μL of virus preservation solution, and to.....
    Document: A confirmed case of COVID-19 is defined as a positive result on high throughput sequencing or real-time reverse-transcriptase-polymerase-chain-reaction (RT-PCR) assay of pharyngeal swab specimens. Samples were collected for extracting SARS-CoV-2 RNA from patients suspected of having SARS-CoV-2 infection as described previously. 14 In brief, the pharyngeal swabs were placed into a collection tube with 150 μL of virus preservation solution, and total RNA was extracted within 2 hours using the respiratory sample RNA isolation kit. Forty μL of cell lysates were transferred into a collection tube followed by vortex for 10 seconds. After standing at room temperature for 10 minutes, the collection tube was centrifugated at 1000 rpm/min for 5 minutes. The suspension was used for RT-PCR assay of SARS-CoV-2 RNA. author/funder, who has granted medRxiv a license to display the preprint in perpetuity.

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