Author: Zhou, Haixia; Zhao, Jincun; Perlman, Stanley
Title: Autocrine Interferon Priming in Macrophages but Not Dendritic Cells Results in Enhanced Cytokine and Chemokine Production after Coronavirus Infection Document date: 2010_10_19
ID: qg541qho_24
Snippet: Viruses and cell cultures. MHV-A59 (MHV) was grown on 17Cl-1 cells, and the titers of the virus on HeLa cells expressing CEACAM1 (carcinoembryonic antigen-related cell adhesion molecule 1), the MHV receptor (51), were determined as previously described (52) . The cells were infected with MHV at a multiplicity of infection (MOI) of 5 or, as a control, with 100 hemagglutination activity units of Sendai virus (SenV) (Cantell strain; Charles River La.....
Document: Viruses and cell cultures. MHV-A59 (MHV) was grown on 17Cl-1 cells, and the titers of the virus on HeLa cells expressing CEACAM1 (carcinoembryonic antigen-related cell adhesion molecule 1), the MHV receptor (51), were determined as previously described (52) . The cells were infected with MHV at a multiplicity of infection (MOI) of 5 or, as a control, with 100 hemagglutination activity units of Sendai virus (SenV) (Cantell strain; Charles River Laboratories, Wilmington, MA). 17Cl-1 cells were cultured in medium consisting of Dulbecco's modified Eagle's medium (DMEM) supplemented with 5% fetal calf serum (FBS), 5% tryptose phosphate, and 1% penicillin-streptomycin.
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