Author: Stenglein, Mark D.; Jacobson, Elliott R.; Wozniak, Edward J.; Wellehan, James F. X.; Kincaid, Anne; Gordon, Marcus; Porter, Brian F.; Baumgartner, Wes; Stahl, Scott; Kelley, Karen; Towner, Jonathan S.; DeRisi, Joseph L.
Title: Ball Python Nidovirus: a Candidate Etiologic Agent for Severe Respiratory Disease in Python regius Document date: 2014_9_9
ID: rb3qdunj_31
Snippet: Attempted isolation of virus in culture. We attempted to isolate the virus in several snake cell lines, including the boa constrictor JK cell line and the viper VSW and VH-2 lines (59, 60). We prepared an inoculum from frozen infected tissue and applied it to these lines. Viral RNA was detectable in the inoculum but disappeared from the culture supernatants as media were replaced over the course of 14 days, and no cytopathic effects were observed.....
Document: Attempted isolation of virus in culture. We attempted to isolate the virus in several snake cell lines, including the boa constrictor JK cell line and the viper VSW and VH-2 lines (59, 60). We prepared an inoculum from frozen infected tissue and applied it to these lines. Viral RNA was detectable in the inoculum but disappeared from the culture supernatants as media were replaced over the course of 14 days, and no cytopathic effects were observed. It is possible that different cells, alternate culture conditions, or inoculum from freshly harvested infected tissue would permit replication.
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