Author: Pechan, Peter; Ardinger, Jeffery; Ketavarapu, Jyothi; Rubin, Hillard; Wadsworth, Samuel C; Scaria, Abraham
Title: Aurintricarboxylic acid increases yield of HSV-1 vectors Document date: 2014_2_19
ID: uk7jr5a4_9
Snippet: Vero-derived V27 cells 45 and HEK-derived 293 cells (HEK-293 cells) 46 were obtained from the Applied Genetic Technologies Corporation (Alachua, FL). Vero cells were purchased from American Type Culture Collection (Manassas, VA). All cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% FBS (HyClone, South Logan, UT) and either 50 mg/ml G418, Geneticin (Invitrogen Life Technologies, Grand Island, NY) for V27 cells or 1.....
Document: Vero-derived V27 cells 45 and HEK-derived 293 cells (HEK-293 cells) 46 were obtained from the Applied Genetic Technologies Corporation (Alachua, FL). Vero cells were purchased from American Type Culture Collection (Manassas, VA). All cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% FBS (HyClone, South Logan, UT) and either 50 mg/ml G418, Geneticin (Invitrogen Life Technologies, Grand Island, NY) for V27 cells or 1% penicillin/streptomycin (Corning Cellgro Mediatech, Manassas, VA) for the other cells. The wtHSV-1 KOS strain and the ICP27-deficient derivatives of the wtHSV-1 KOS strain: vectors d27-1, 47 rHSV-rep2/cap2, and rHSV-EGFP, 11 and their producer ICP27-complementing V27 cell line were obtained from the Applied Genetic Technologies Corporation. The wtHSV-1 McIntyre strain was purchased from Advanced Biotechnologies (Columbia MD).
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