2017 ACVIM Forum Research Abstract Program

Formalin-fixed paraffin wax-embedded samples of 30 canine urinary bladders with transitional cell carcinoma, 10 canine urinary bladders with cystitis and 10 normal canine urinary bladders were obtained from the University of Tennessee Department of Pathobiology histologic archives for use in this study. Staining for PDGFR-b, VEGFR2, and KIT was carried out using standard immunohistochemical procedures developed for use in formalin fixed tissues. Staining intensity was evaluated at 209 in representative sections. Overall staining intensity was scored from 0 to 3 for the entire section. Immunohistochemical staining (% cells stained) was evaluated over ten 409 fields and given a score from 1 to 4. A final scoring product was calculated by multiplying the intensity score by the immunohistochemical staining score A significant number of TCC samples (30/30) expressed PDGFR-b compared to cystitis (5/10) and normal bladder samples (3/10). A large number of the TCC samples (11/30) also had mild to moderate staining for KIT compared to cystitis (0/10) and normal bladder (0/10) samples, but the difference was not statistically significant.