Attractiveness of an Aggregation Pheromone Lure and Chicken Droppings to Adults and Larvae of Alphitobius diaperinus (Coleoptera: Tenebrionidae)

Singh, N.; Johnson, D. T.

Laboratory Two-Choice Pitfall Bioassay. The pheromone lure consisted of Þve compounds: (R)-(ϩ)-limonene (97%), (E)-␤-ocimene (98%), (S)-(ϩ)-linalool (98%), (R)-(ϩ)-daucene (98%), and 2-nonanone (99%) in a 9.6: 60.0: 9.6: 17.3: 3.5 ratios, respectively (Bartelt et al. 2009 ). The two commercial components, 2-nonanone and (R)-(ϩ)-limonene were purchased from Aldrich, Milwaukee, WI. (E)-␤-ocimene, (S)-(ϩ)-linalool, and (R)-(ϩ)-daucene were prepared in the USDAÐARS laboratory at Peoria, IL, by the procedures described in Bartelt et al. (2009) . Pheromone lure doses ranging from 1 to 100 g were tested to determine responses of lesser mealworm adults and larvae. Because 20 g of pheromone lure was found to be more attractive in preliminary bioassays, this amount of pheromone lure, or individual components of pheromone lure was used in subsequent bioassays. Emission characteristics of the pheromone lure components placed on rubber septa were measured in the laboratory by the methods of Bartelt et al. (2009) .