Author: Sun, Ying; Xiao, ShaoBo; Wang, Dang; Luo, Rui; Li, Bin; Chen, HuanChun; Fang, LiuRong
Title: Cellular membrane cholesterol is required for porcine reproductive and respiratory syndrome virus entry and release in MARC-145 cells Document date: 2011_12_16
ID: zrsnahi7_11
Snippet: To determine if treatment with MβCD reduces the cellular cholesterol content, MARC-145 cells in 24-well plates were washed three times with cold PBS, and then incubated for 1 h with serum-free DMEM (control cells) or various concentrations of MβCD (treated cells). After three additional washes with PBS, cells were harvested and the cholesterol content was measured using the Amplex Red Cholesterol Assay Kit (Molecular Probes, Oregon, USA), as di.....
Document: To determine if treatment with MβCD reduces the cellular cholesterol content, MARC-145 cells in 24-well plates were washed three times with cold PBS, and then incubated for 1 h with serum-free DMEM (control cells) or various concentrations of MβCD (treated cells). After three additional washes with PBS, cells were harvested and the cholesterol content was measured using the Amplex Red Cholesterol Assay Kit (Molecular Probes, Oregon, USA), as directed by the manufacturer.
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