Selected article for: "deep sequencing and RNA virus"
Author: Drexler, Jan Felix; Corman, Victor Max; Müller, Marcel Alexander; Maganga, Gael Darren; Vallo, Peter; Binger, Tabea; Gloza-Rausch, Florian; Rasche, Andrea; Yordanov, Stoian; Seebens, Antje; Oppong, Samuel; Sarkodie, Yaw Adu; Pongombo, Célestin; Lukashev, Alexander N.; Schmidt-Chanasit, Jonas; Stöcker, Andreas; Carneiro, Aroldo José Borges; Erbar, Stephanie; Maisner, Andrea; Fronhoffs, Florian; Buettner, Reinhard; Kalko, Elisabeth K.V.; Kruppa, Thomas; Franke, Carlos Roberto; Kallies, René; Yandoko, Emmanuel R.N.; Herrler, Georg; Reusken, Chantal; Hassanin, Alexandre; Krüger, Detlev H.; Matthee, Sonja; Ulrich, Rainer G.; Leroy, Eric M.; Drosten, Christian
Title: Bats host major mammalian paramyxoviruses
  • Document date: 2012_4_24
    • ID: yw028ohl_24
    Snippet: Like almost all PVs currently contained in GenBank, our novel viruses were identified by RT-PCR and sequencing. PCR primers have an inherent bias due to their sequence specificity. We have applied a large range of published and own primer sets to compensate for this bias. Some of these have been thoroughly validated on clinical panels from a large range of hosts, confirming high sensitivity over a large genetic range of viruses 31 . More recent s.....
    Document: Like almost all PVs currently contained in GenBank, our novel viruses were identified by RT-PCR and sequencing. PCR primers have an inherent bias due to their sequence specificity. We have applied a large range of published and own primer sets to compensate for this bias. Some of these have been thoroughly validated on clinical panels from a large range of hosts, confirming high sensitivity over a large genetic range of viruses 31 . More recent studies have suggested to improve the detection of novel viruses by hypothesisfree approaches such as random cDNA amplification from serum followed by deep sequencing 32 . Inclusion of such an approach in our study indeed revealed novel viral sequences that should be investigated further, but did not yield any PV. Low serum concentrations of PV RNA may have prevented detection, reminding us that hypothesis-free virus testing may not be sufficiently sensitive to enable comprehensive detection of viral flora 32, 33 .

    Search related documents:
    Co phrase search for related documents
    • cdna amplification and high sensitivity: 1, 2, 3
    • cdna amplification and large range: 1, 2
    • clinical panel and deep sequencing: 1
    • clinical panel and high sensitivity: 1, 2, 3, 4, 5, 6, 7
    • comprehensive detection and deep sequencing: 1
    • comprehensive detection and high sensitivity: 1, 2, 3, 4, 5, 6
    • deep sequencing and detection prevent: 1
    • deep sequencing and genetic range: 1, 2
    • deep sequencing and high sensitivity: 1, 2, 3, 4, 5
    • deep sequencing and host large range: 1, 2
    • deep sequencing and large range: 1, 2, 3
    • detection prevent and high sensitivity: 1, 2, 3, 4, 5
    • genetic range and high sensitivity: 1
    • high sensitivity and inherent bias: 1
    • high sensitivity and large range: 1, 2, 3, 4, 5, 6
    • host large range and large range: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10