Author: Drexler, Jan Felix; Corman, Victor Max; Müller, Marcel Alexander; Maganga, Gael Darren; Vallo, Peter; Binger, Tabea; Gloza-Rausch, Florian; Rasche, Andrea; Yordanov, Stoian; Seebens, Antje; Oppong, Samuel; Sarkodie, Yaw Adu; Pongombo, Célestin; Lukashev, Alexander N.; Schmidt-Chanasit, Jonas; Stöcker, Andreas; Carneiro, Aroldo José Borges; Erbar, Stephanie; Maisner, Andrea; Fronhoffs, Florian; Buettner, Reinhard; Kalko, Elisabeth K.V.; Kruppa, Thomas; Franke, Carlos Roberto; Kallies, René; Yandoko, Emmanuel R.N.; Herrler, Georg; Reusken, Chantal; Hassanin, Alexandre; Krüger, Detlev H.; Matthee, Sonja; Ulrich, Rainer G.; Leroy, Eric M.; Drosten, Christian
Title: Bats host major mammalian paramyxoviruses Document date: 2012_4_24
ID: yw028ohl_32
Snippet: Next-generation sequencing. Products of random cDNA amplification were loaded on 1.2% agarose gels. Primer dimers and large fragments ( > 700 bp) were removed, and amplicons were extracted from agarose gels. Fragments were endrepaired and a 454 sequencing library was constructed according to the GS Junior Rapid Library Preparation protocol (Roche, Penzberg, Germany). Emulsion PCR and sequencing reaction were performed as recommended by the manufa.....
Document: Next-generation sequencing. Products of random cDNA amplification were loaded on 1.2% agarose gels. Primer dimers and large fragments ( > 700 bp) were removed, and amplicons were extracted from agarose gels. Fragments were endrepaired and a 454 sequencing library was constructed according to the GS Junior Rapid Library Preparation protocol (Roche, Penzberg, Germany). Emulsion PCR and sequencing reaction were performed as recommended by the manufacturer. Primer sequences were trimmed from each read, and all reads were aligned against the NCBI virus database using the tblastx local alignment algorithm in Geneious. All hits were scored and alignments with lengths less than 50 amino acids and a bit-score less than 40 were excluded.
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