2017 ACVIM Forum Research Abstract Program

Ten horses, 5 PPID + and 5 PPID À (non-PPID) horses were enrolled in the study during the autumn time-period. On Day 0, 5 mL of whole blood was collected from each horse into each of six EDTA tubes and immediately chilled. One tube was centrifuged within 15 minutes of collection (time 0) followed by centrifugation of one tube from each horse at 4, 8, 12, 24 and 36 hours following collection. At each time-point, plasma was centrifuged and separated into 1.5 mL polypropylene tubes and stored at À80°C. Plasma was shipped frozen with cold packs overnight to the Animal Health Diagnostic Center, Cornell University, Ithaca, NY for analysis. The percent change from baseline (PCFB) was reported to standardize the data due to differing baseline values. Mean PCFB, standard deviation and 95% confidence intervals (CI) were reported. The null hypothesis that there was no time effect and was tested with a mixed model repeated measures methodology with subject as a random effect. P-values < 0.05 were considered statistically significant.