Title: Biosynthesis and processing of ribophorins in the endoplasmic reticulum Document date: 1984_9_1
ID: tuf1ih4g_17
Snippet: Detergent extracts of rat liver rough microsomes were iodinated by the chloramine T procedure as previously described (34) . After immuneprecipitation of the ribophorins, the beads were boiled in 2% SDS and centrifuged. Supernatants diluted with 1 M citrate-phosphate buffer (pH 5.0 or pH 6.4) to a final concentration of 50 mM were incubated with endogiycosidase H (8 x l0 -4 U/ml) or with a crude preparation of Endoglycoside D (38) for l h at 37"C.....
Document: Detergent extracts of rat liver rough microsomes were iodinated by the chloramine T procedure as previously described (34) . After immuneprecipitation of the ribophorins, the beads were boiled in 2% SDS and centrifuged. Supernatants diluted with 1 M citrate-phosphate buffer (pH 5.0 or pH 6.4) to a final concentration of 50 mM were incubated with endogiycosidase H (8 x l0 -4 U/ml) or with a crude preparation of Endoglycoside D (38) for l h at 37"C at pH 5.0 or 6.4, respectively. Samples were then analyzed by PAGE (10%) followed by autoradiography.
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