Author: Stenglein, Mark D.; Jacobson, Elliott R.; Wozniak, Edward J.; Wellehan, James F. X.; Kincaid, Anne; Gordon, Marcus; Porter, Brian F.; Baumgartner, Wes; Stahl, Scott; Kelley, Karen; Towner, Jonathan S.; DeRisi, Joseph L.
Title: Ball Python Nidovirus: a Candidate Etiologic Agent for Severe Respiratory Disease in Python regius Document date: 2014_9_9
ID: rb3qdunj_28
Snippet: To independently confirm the metagenomic sequencing results, we used quantitative RT-PCR (qRT-PCR) to measure viral RNA levels in these samples. Viral RNA was detected in all affected animals but not in control animals (Fig. 6A) . Thus, detection of viral RNA by both metagenomic sequencing and qRT-PCR perfectly correlated with disease status, though larger studies will be necessary to confirm this association......
Document: To independently confirm the metagenomic sequencing results, we used quantitative RT-PCR (qRT-PCR) to measure viral RNA levels in these samples. Viral RNA was detected in all affected animals but not in control animals (Fig. 6A) . Thus, detection of viral RNA by both metagenomic sequencing and qRT-PCR perfectly correlated with disease status, though larger studies will be necessary to confirm this association.
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