Selected article for: "dose dependent manner and MÎCD treatment"
Author: Sun, Ying; Xiao, ShaoBo; Wang, Dang; Luo, Rui; Li, Bin; Chen, HuanChun; Fang, LiuRong
Title: Cellular membrane cholesterol is required for porcine reproductive and respiratory syndrome virus entry and release in MARC-145 cells
  • Document date: 2011_12_16
    • ID: zrsnahi7_26
    Snippet: It is known that the plasma membrane lipid composition plays a key role in the infection by many enveloped viruses [2428]. To investigate the role of plasma membrane cholesterol in the infection of PRRSV, MARC-145 cells were mock-treated or treated with various concentrations of MβCD and infected with PRRSV strain CH-1a at 10 4.5 TCID 50 per well. After incubation for 36 h, cell suspensions were harvested and virus production was determined b.....
    Document: It is known that the plasma membrane lipid composition plays a key role in the infection by many enveloped viruses [2428]. To investigate the role of plasma membrane cholesterol in the infection of PRRSV, MARC-145 cells were mock-treated or treated with various concentrations of MβCD and infected with PRRSV strain CH-1a at 10 4.5 TCID 50 per well. After incubation for 36 h, cell suspensions were harvested and virus production was determined by TCID 50 assay on MARC-145 cells. As shown in Figure 2A , MβCD treatment inhibited the infection of PRRSV strain CH-1a in a dose-dependent manner, suggesting that cholesterol is necessary for PRRSV replication in MARC-145 cells. Moreover, treatment with 10 mmol L 1 MβCD efficiently reduced virus titer to 10 2.75 TCID 50 mL 1 compared with that of 10 6.5 TCID 50 mL 1 in mock-treated cells.

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