Author: Sun, Ying; Xiao, ShaoBo; Wang, Dang; Luo, Rui; Li, Bin; Chen, HuanChun; Fang, LiuRong
Title: Cellular membrane cholesterol is required for porcine reproductive and respiratory syndrome virus entry and release in MARC-145 cells Document date: 2011_12_16
ID: zrsnahi7_31
Snippet: The effect of cholesterol depletion on virus attachment was defined, but next we determined whether penetration of PRRSV into MARC-145 cell was also affected by cholesterol depletion. To this end, MARC-145 cells in 24-well plates were infected with 10 μg biotinylated-PRRSV per well and were mock-treated or treated with MβCD or MβCD-cholesterol. During the treatment, virions attached on these cells completed the penetration process and were det.....
Document: The effect of cholesterol depletion on virus attachment was defined, but next we determined whether penetration of PRRSV into MARC-145 cell was also affected by cholesterol depletion. To this end, MARC-145 cells in 24-well plates were infected with 10 μg biotinylated-PRRSV per well and were mock-treated or treated with MβCD or MβCD-cholesterol. During the treatment, virions attached on these cells completed the penetration process and were detected by confocal microscopy. No significant differences were observed between various groups (data not shown), suggesting that MβCD treatment has little effect on the second stage of virus entry. Altogether, these data indicated that cellular membrane cholesterol was required for PRRSV entry into MARC-145 cells, and its depletion by MβCD mainly affects virus attachment, rather than penetration.
Search related documents:
Co phrase search for related documents- biotinylated prrsv and mβcd cholesterol: 1
- cell attach and membrane cholesterol: 1
- cellular membrane cholesterol and cholesterol depletion: 1, 2, 3, 4
- cellular membrane cholesterol and confocal microscopy: 1
- cellular membrane cholesterol and membrane cholesterol: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
- cellular membrane cholesterol and mβcd cholesterol: 1, 2, 3, 4, 5
- cholesterol depletion affect and membrane cholesterol: 1, 2, 3, 4, 5
- cholesterol depletion affect and mβcd cholesterol: 1, 2, 3
- cholesterol depletion and confocal microscopy: 1, 2
- cholesterol depletion and membrane cholesterol: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- cholesterol depletion and mβcd cholesterol: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24
- confocal microscopy and membrane cholesterol: 1
- confocal microscopy and mβcd cholesterol: 1
- μg biotinylated prrsv and mβcd cholesterol: 1
- little effect and membrane cholesterol: 1, 2
- little effect and mβcd cholesterol: 1, 2
- membrane cholesterol and mβcd cholesterol: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23
Co phrase search for related documents, hyperlinks ordered by date