Author: Stenglein, Mark D.; Jacobson, Elliott R.; Wozniak, Edward J.; Wellehan, James F. X.; Kincaid, Anne; Gordon, Marcus; Porter, Brian F.; Baumgartner, Wes; Stahl, Scott; Kelley, Karen; Towner, Jonathan S.; DeRisi, Joseph L.
Title: Ball Python Nidovirus: a Candidate Etiologic Agent for Severe Respiratory Disease in Python regius Document date: 2014_9_9
ID: rb3qdunj_43
Snippet: (iv) Collections 5 and 6. Two BPs, one from private collection 5 in Florida (BP9) and the other from private collection 6 in Oklahoma (BP10), were submitted to an exotic animal practice for evaluation due to signs of respiratory disease and anorexia. BP9 was part of a small breeding collection where very few sick animals were previously observed. BP9 was euthanized, and the following tissues were collected during necropsy, fixed in NBF, and proce.....
Document: (iv) Collections 5 and 6. Two BPs, one from private collection 5 in Florida (BP9) and the other from private collection 6 in Oklahoma (BP10), were submitted to an exotic animal practice for evaluation due to signs of respiratory disease and anorexia. BP9 was part of a small breeding collection where very few sick animals were previously observed. BP9 was euthanized, and the following tissues were collected during necropsy, fixed in NBF, and processed for light microscopy: trachea, lung, kidney, salivary gland, liver, brain, and small intestine. Portions of trachea, liver, and lung were collected, frozen at Ϫ20°C, and subsequently submitted for molecular diagnostics. Six-micrometer sections of paraffin-embedded tissues were stained with hematoxylin and eosin.
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