Author: Sun, Ying; Xiao, ShaoBo; Wang, Dang; Luo, Rui; Li, Bin; Chen, HuanChun; Fang, LiuRong
Title: Cellular membrane cholesterol is required for porcine reproductive and respiratory syndrome virus entry and release in MARC-145 cells Document date: 2011_12_16
ID: zrsnahi7_5
Snippet: PRRSV strain CH-1a, the first field isolate from China, was kindly provided by Dr. Tong G. Z. (Shanghai Veterinary Research Institute, Shanghai, China). PRRSV strain WUH3, a highly pathogenic North American-type PRRSV, was isolated in 2007 [31] . MARC-145 cells were cultured and maintained in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified 5% CO 2 incubator. Methyl-β-cyclodextrin (M.....
Document: PRRSV strain CH-1a, the first field isolate from China, was kindly provided by Dr. Tong G. Z. (Shanghai Veterinary Research Institute, Shanghai, China). PRRSV strain WUH3, a highly pathogenic North American-type PRRSV, was isolated in 2007 [31] . MARC-145 cells were cultured and maintained in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified 5% CO 2 incubator. Methyl-β-cyclodextrin (MβCD) and water-soluble cholesterol were purchased from Sigma (St Louis, USA).
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