Selected article for: "blue method and brilliant blue method"
Author: Lu, Yan; Jiang, Yun; Ling, Lijun; Zhang, Yunyi; Li, Hong; Chen, Daofeng
Title: Beneficial effects of Houttuynia cordata polysaccharides on “two-hit” acute lung injury and endotoxic fever in rats associated with anti-complementary activities
  • Document date: 2017_12_8
    • ID: q4r36tpw_23
    Snippet: Bronchoalveolar lavage fluid (BALF) was collected immediately after sacrifice. The lobes of left lung were lavaged with a total volume of 4 mL saline at 4°C. BALF was centrifuged at 111 × g for 10 min. The cell-free supernatant was collected and stored at −20°C for further assays. Total cell counts were performed on the Neubauer's counting chamber. The leukocyte classifications were examined by counting at least 100 cells/smear prepared usin.....
    Document: Bronchoalveolar lavage fluid (BALF) was collected immediately after sacrifice. The lobes of left lung were lavaged with a total volume of 4 mL saline at 4°C. BALF was centrifuged at 111 × g for 10 min. The cell-free supernatant was collected and stored at −20°C for further assays. Total cell counts were performed on the Neubauer's counting chamber. The leukocyte classifications were examined by counting at least 100 cells/smear prepared using cytospin and Wright-Giemsa staining. The obtained values represented the percentage of neutrophils or lymphocytes in total leukocytes 28 . The concentration of the total protein was determined by the Coomassie brilliant blue method 29 . The assays of superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were carried out using commercial kits.

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