Author: Xing, Liang; Sun, Feng; Wang, Zhendong; Li, Yan; Yang, Zhifang; Wang, Fengshan; Zhai, Guangxi; Tan, Haining
Title: Characterization and bioactivity of self-assembled anti-angiogenic chondroitin sulfate-ES2-AF nanoparticle conjugate Document date: 2019_4_10
ID: x8f598x2_60
Snippet: In the pharmacokinetic study of peptide drugs, several commonly used methods include isotopic tracer method, 61 ELISA, 10 liquid chromatography-mass spectrometry (LC-MS), 62, 63 and fluorescence labeling. At present, the US FDA uses the pharmacokinetic data obtained by radioisotope determination of peptide drugs for the effective evaluation of the safety of the drugs. However, polypeptides labeled with radioisotopes, such as 125 I are prone to be.....
Document: In the pharmacokinetic study of peptide drugs, several commonly used methods include isotopic tracer method, 61 ELISA, 10 liquid chromatography-mass spectrometry (LC-MS), 62, 63 and fluorescence labeling. At present, the US FDA uses the pharmacokinetic data obtained by radioisotope determination of peptide drugs for the effective evaluation of the safety of the drugs. However, polypeptides labeled with radioisotopes, such as 125 I are prone to be labeled off-label during the experiment, as they are unable to accurately reflect the concentration of the polypeptide drugs from the measured radioactivity. This method also has the shortcomings of radioactive contamination, thus limiting its application in clinical pharmacokinetics research. Although ELISA method is simple and convenient to operate, it is necessary to develop related antibody-coated kits. The preparation period of the kit is not long and it is not susceptible to interference by biological endogenous and exogenous substances during the measurement process. The LC/MS has the advantages of good specificity and high sensitivity. However, plasma samples contain a large amount of salts and endogenous proteins, which may interfere with the measurement process. Fluorescence labeling has been used by increasing number of people because of its mild reaction conditions and simple detection method.
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