Selected article for: "quantitative PCR and viral RNA"
Author: Stenglein, Mark D.; Jacobson, Elliott R.; Wozniak, Edward J.; Wellehan, James F. X.; Kincaid, Anne; Gordon, Marcus; Porter, Brian F.; Baumgartner, Wes; Stahl, Scott; Kelley, Karen; Towner, Jonathan S.; DeRisi, Joseph L.
Title: Ball Python Nidovirus: a Candidate Etiologic Agent for Severe Respiratory Disease in Python regius
  • Document date: 2014_9_9
    • ID: rb3qdunj_52
    Snippet: Quantitative PCR. To measure relative levels of viral RNA in samples, RNA was extracted and reverse transcribed as described above for library preparation. cDNA was diluted 1:10 in 10 mM Tris-0.1 mM EDTA, pH 8. qPCR reaction mixtures contained 10 mM Tris, pH 8.6, 50 mM KCl, 1.5 mM MgCl2, 5% glycerol, 0.08% IGEPAL CA-630, 0.05% Tween 20, 0.2 mM (each) dNTP, 1Ï« Sybr green (Life Technologies), 0.5 U Taq polymerase, and 0.25 M (each) primer (see Tab.....
    Document: Quantitative PCR. To measure relative levels of viral RNA in samples, RNA was extracted and reverse transcribed as described above for library preparation. cDNA was diluted 1:10 in 10 mM Tris-0.1 mM EDTA, pH 8. qPCR reaction mixtures contained 10 mM Tris, pH 8.6, 50 mM KCl, 1.5 mM MgCl2, 5% glycerol, 0.08% IGEPAL CA-630, 0.05% Tween 20, 0.2 mM (each) dNTP, 1Ï« Sybr green (Life Technologies), 0.5 U Taq polymerase, and 0.25 M (each) primer (see Table S1 in the supplemental material). Viral RNA levels were normalized to levels of cellular GAPDH (see Table S1 ). Reaction efficiencies were determined using serial dilutions of templates (79) .

    Search related documents:
    Co phrase search for related documents
    • library preparation and supplemental material: 1, 2, 3, 4
    • library preparation and supplemental material Table S1: 1, 2, 3
    • library preparation and supplemental material Table S1 primer: 1
    • library preparation and Table S1: 1, 2, 3, 4
    • library preparation and viral RNA level: 1
    • qPCR reaction and reaction efficiency: 1, 2, 3, 4, 5
    • qPCR reaction and relative level: 1, 2
    • qPCR reaction and RNA level: 1, 2
    • qPCR reaction and serial dilution: 1, 2, 3, 4, 5, 6
    • qPCR reaction and supplemental material: 1
    • qPCR reaction and supplemental material Table S1: 1
    • qPCR reaction and Table S1: 1, 2, 3, 4, 5
    • qPCR reaction and viral RNA level: 1
    • qPCR reaction mixture and relative level: 1, 2
    • reaction efficiency and serial dilution: 1