Title: Biosynthesis and processing of ribophorins in the endoplasmic reticulum Document date: 1984_9_1
ID: tuf1ih4g_15
Snippet: Cell-free Protein Synthesis: Wheat germ extracts (35) containing 12.5 ~Ci of [3SS]methionine and 0.03 A26o U of mRNA in 0.025 ml were incubated at 25"C for 90 min. Dog pancreas microsomal membranes, prepared as described by Shields and Blobel (52) , were added to a final concentration of 2 A26o U/ml. Samples from translation mixtures (0.2 ml) were boiled in 2% SDS, centrifuged (2 min at 15,000 g), and diluted fourfold with solution A. Immunepreci.....
Document: Cell-free Protein Synthesis: Wheat germ extracts (35) containing 12.5 ~Ci of [3SS]methionine and 0.03 A26o U of mRNA in 0.025 ml were incubated at 25"C for 90 min. Dog pancreas microsomal membranes, prepared as described by Shields and Blobel (52) , were added to a final concentration of 2 A26o U/ml. Samples from translation mixtures (0.2 ml) were boiled in 2% SDS, centrifuged (2 min at 15,000 g), and diluted fourfold with solution A. Immuneprecipitation was carried out as described above.
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