Selected article for: "cell line and pituitary cell line"

Title: Biosynthesis and processing of ribophorins in the endoplasmic reticulum
  • Document date: 1984_9_1
  • ID: tuf1ih4g_14
    Snippet: Free and membrane-bound polysomes were prepared from cl-9 hepatocytes by a modification of the procedure of Ramsey and Steele (43) . Polysome pellets were rinsed with sterile water and the RNA was extracted with 5 M guanidine thiocyanate as described above. Poly A ÷ mRNA was purified by chromatography on oligo-dT cellulose (2) . Preparations of messenger RNA from the pituitary cell line GH 3. l were fractionated on a methyl mercury gel (40) to e.....
    Document: Free and membrane-bound polysomes were prepared from cl-9 hepatocytes by a modification of the procedure of Ramsey and Steele (43) . Polysome pellets were rinsed with sterile water and the RNA was extracted with 5 M guanidine thiocyanate as described above. Poly A ÷ mRNA was purified by chromatography on oligo-dT cellulose (2) . Preparations of messenger RNA from the pituitary cell line GH 3. l were fractionated on a methyl mercury gel (40) to enrich for ribophorin encoding molecules.

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