Author: Chu, Ruiyin; Reczek, David; Brondyk, William
Title: Capture-stabilize approach for membrane protein SPR assays Document date: 2014_12_8
ID: ueofl0wn_14
Snippet: Stabilization of hCXCR5. Ni-affinity captured hCXCR5 receptor protein was stabilized with limited cross-linking by flow over the mixtures of 20 mM EDC and 5 mM NHS for 7 min. Subsequently, excess cross-linkers were quenched with 1M ethanolamine for 10 min. The surface stabilized receptor chip was primed with running buffer before subsequent assay steps......
Document: Stabilization of hCXCR5. Ni-affinity captured hCXCR5 receptor protein was stabilized with limited cross-linking by flow over the mixtures of 20 mM EDC and 5 mM NHS for 7 min. Subsequently, excess cross-linkers were quenched with 1M ethanolamine for 10 min. The surface stabilized receptor chip was primed with running buffer before subsequent assay steps.
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