Selected article for: "crude peptide and peptide segment"

Author: Harmand, Thibault J.; Pattabiraman, Vijaya R.; Bode, Jeffrey W.
Title: Chemical Synthesis of the Highly Hydrophobic Antiviral Membrane-Associated Protein IFITM3 and Modified Variants
  • Document date: 2017_9_7
  • ID: so0685rt_42
    Snippet: The resin was subjected to automated Fmoc SPPS using standard procedures as described in the General Methods. After N-terminal deprotection the peptide sulfurylide was cleaved from the resin and the filtrate was concentrated under vacuum. The peptide segment was precipitated with Et 2 O and after centrifugation and drying, afforded the crude peptide (214 mg). The crude peptide was placed in a vial under N 2 atmosphere and could be kept for months.....
    Document: The resin was subjected to automated Fmoc SPPS using standard procedures as described in the General Methods. After N-terminal deprotection the peptide sulfurylide was cleaved from the resin and the filtrate was concentrated under vacuum. The peptide segment was precipitated with Et 2 O and after centrifugation and drying, afforded the crude peptide (214 mg). The crude peptide was placed in a vial under N 2 atmosphere and could be kept for months at 4 °C.

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