Selected article for: "amide polystyrene resin rink and DMF NMM HATU"
Author: Harmand, Thibault J.; Pattabiraman, Vijaya R.; Bode, Jeffrey W.
Title: Chemical Synthesis of the Highly Hydrophobic Antiviral Membrane-Associated Protein IFITM3 and Modified Variants
  • Document date: 2017_9_7
    • ID: so0685rt_9
    Snippet: Native sequence (without the initial signal peptide): NpecOpr-S2-ValKA 2 was synthesized on rink amide polystyrene resin (0.36 mmol/g, scale: 1.0 g, 0.36 mmol) using protected Val-KA with 0.25 mmol/g loading. After automated Fmoc SPPS, the Nterminal Fmoc was removed, and Npec-Opr-OH was manually coupled using HATU and NMM in DMF 4 h followed by washing with DMF and CH 2 Cl 2 . The dried resin was subjected to cleavage using (95:2.5:2.5) TFA:DODT:.....
    Document: Native sequence (without the initial signal peptide): NpecOpr-S2-ValKA 2 was synthesized on rink amide polystyrene resin (0.36 mmol/g, scale: 1.0 g, 0.36 mmol) using protected Val-KA with 0.25 mmol/g loading. After automated Fmoc SPPS, the Nterminal Fmoc was removed, and Npec-Opr-OH was manually coupled using HATU and NMM in DMF 4 h followed by washing with DMF and CH 2 Cl 2 . The dried resin was subjected to cleavage using (95:2.5:2.5) TFA:DODT:H 2 O, filtered and the filtrate was evaporated under vacuum. The peptide segment was precipitated with Et 2 O; centrifugation and drying afforded the crude peptide (980 mg).

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