Author: Chu, Ruiyin; Reczek, David; Brondyk, William
Title: Capture-stabilize approach for membrane protein SPR assays Document date: 2014_12_8
ID: ueofl0wn_13
Snippet: Reversed peptide-based Biacore assay. C-terminal biotinylated peptide (MNYPLTLEMD LENLEDLFWE LDRLDNYNDT SLVENHLCPA TEGPLMASFK www.nature.com/scientificreports Capturing hCXCR5 on Biacore sensor chip surface. For direct NTA chip capture, following 1 min injection of 0.5 mM NiCl2, purified hCXCR5 receptor at concentration of 50 ng/ml diluted in HBS-N buffer was injected with a flow rate of 5 ml/min to achieve capture level between 300-700 resonance.....
Document: Reversed peptide-based Biacore assay. C-terminal biotinylated peptide (MNYPLTLEMD LENLEDLFWE LDRLDNYNDT SLVENHLCPA TEGPLMASFK www.nature.com/scientificreports Capturing hCXCR5 on Biacore sensor chip surface. For direct NTA chip capture, following 1 min injection of 0.5 mM NiCl2, purified hCXCR5 receptor at concentration of 50 ng/ml diluted in HBS-N buffer was injected with a flow rate of 5 ml/min to achieve capture level between 300-700 resonance units (RU), depending on experiment purpose.
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