Author: Abdelhamid, Ahmed G.; Esaam, Aliaa; Hazaa, Mahmoud M.
Title: Cell free preparations of probiotics exerted antibacterial and antibiofilm activities against multidrug resistant E. coli Document date: 2018_3_12
ID: w98pwltg_10
Snippet: The E. coli test isolates were activated on trypticase soy agar (Oxoid) for 24 h at 37°C. Suspension of each test bacteria of 10 9 CFU/ml was prepared by growing each bacterium in trypticase soy broth (Oxoid) for 24 h at 37°C. The agar diffusion method was used to determine the inhibition zone of the test bacteria (Cruz et al., 2001) . First, a bottom layer (10 ml) of TSA was prepared in the petri-dish. Secondly, a top layer of molten and coole.....
Document: The E. coli test isolates were activated on trypticase soy agar (Oxoid) for 24 h at 37°C. Suspension of each test bacteria of 10 9 CFU/ml was prepared by growing each bacterium in trypticase soy broth (Oxoid) for 24 h at 37°C. The agar diffusion method was used to determine the inhibition zone of the test bacteria (Cruz et al., 2001) . First, a bottom layer (10 ml) of TSA was prepared in the petri-dish. Secondly, a top layer of molten and cooled TSA (5 ml) mixed with each test bacteria suspension (10 9 CFU/ml) was poured on the bottom layer. Five 6 mm in diameter wells were prepared in each plate, and 100 ml of the probiotic CFSM was introduced in each well. The test bacteria were incubated for 24 h at 37°C and the inhibition zones were measured in millimetres.
Search related documents:
Co phrase search for related documents- agar diffusion method and inhibition zone: 1
- diffusion method and inhibition zone: 1, 2, 3, 4, 5
- diffusion method and soy agar: 1
- diffusion method and test bacteria: 1
- inhibition zone and test bacteria: 1
- inhibition zone and test bacteria suspension: 1
Co phrase search for related documents, hyperlinks ordered by date