Author: Lim, Jaegyun; Jeon, Seunghyun; Shin, Hyun-Young; Kim, Moon Jung; Seong, Yu Min; Lee, Wang Jun; Choe, Kang-Won; Kang, Yu Min; Lee, Baeckseung; Park, Sang-Joon
Title: Case of the Index Patient Who Caused Tertiary Transmission of Coronavirus Disease 2019 in Korea: the Application of Lopinavir/Ritonavir for the Treatment of COVID-19 Pneumonia Monitored by Quantitative RT-PCR Document date: 2020_2_14
ID: t159ctrv_6
Snippet: The initial viral load could not be measured because real-time PCR was not available when the patient was diagnosed. So we measured viral loads using quantitative reverse transcription (RT)-PCR since Jan. 31, 2020. Viral RNA was extracted from the sputum using QIAamp viral RNA mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. All quantitative real-time PCR amplifications were performed using Quantstudio 1 (Applied B.....
Document: The initial viral load could not be measured because real-time PCR was not available when the patient was diagnosed. So we measured viral loads using quantitative reverse transcription (RT)-PCR since Jan. 31, 2020. Viral RNA was extracted from the sputum using QIAamp viral RNA mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. All quantitative real-time PCR amplifications were performed using Quantstudio 1 (Applied Biosystems, Foster City, CA, USA) and PowerCheckâ„¢ SARS-CoV-2 Real-Time PCR kit (KogeneBiotech, Seoul, Korea).
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