Author: Maceyka, Michael; Machamer, Carolyn E.
Title: Ceramide Accumulation Uncovers a Cycling Pathway for the cis-Golgi Network Marker, Infectious Bronchitis Virus M Protein Document date: 1997_12_15
ID: wekvet6f_17
Snippet: We next asked if PDMP mislocalized other Golgi markers. Cells were infected and treated as described above and then stained with antibodies to two integral membrane proteins of the Golgi stack, giantin (Linstedt and Hauri, 1993) and mannosidase II (Man II; Moremen and Robbins, 1991; Velasco et al., 1993) . Treatment of cells with 100 M PDMP for 1 h had no effect on the localization of either protein (Fig. 4) , suggesting that the morphology of th.....
Document: We next asked if PDMP mislocalized other Golgi markers. Cells were infected and treated as described above and then stained with antibodies to two integral membrane proteins of the Golgi stack, giantin (Linstedt and Hauri, 1993) and mannosidase II (Man II; Moremen and Robbins, 1991; Velasco et al., 1993) . Treatment of cells with 100 M PDMP for 1 h had no effect on the localization of either protein (Fig. 4) , suggesting that the morphology of the Golgi was not greatly altered. We also examined the localization of ⤠-COP, a peripheral membrane protein of the stack and CGN involved in vesicular traffic (Oprins et al., 1993) . As seen in Fig. 4 , the distribution of ⤠-COP appeared unaffected in treated cells.
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