Author: Abdelhamid, Ahmed G.; Esaam, Aliaa; Hazaa, Mahmoud M.
Title: Cell free preparations of probiotics exerted antibacterial and antibiofilm activities against multidrug resistant E. coli Document date: 2018_3_12
ID: w98pwltg_8
Snippet: The preparation of CFSM of each probiotic strain was performed as described previously (Bayoumi and Griffiths, 2012) . Briefly; overnight cultures of the six probiotic strains grown in MRS broth at 37°C were diluted 1:100 with fresh medium and allowed to grow under same conditions to an optical density at 600 nm of 1.6 ($1 Â 10 8 cells/ml), the cells were then removed using centrifugation at 6000g at 4°C for 10 min. The supernatant was filter-.....
Document: The preparation of CFSM of each probiotic strain was performed as described previously (Bayoumi and Griffiths, 2012) . Briefly; overnight cultures of the six probiotic strains grown in MRS broth at 37°C were diluted 1:100 with fresh medium and allowed to grow under same conditions to an optical density at 600 nm of 1.6 ($1 Â 10 8 cells/ml), the cells were then removed using centrifugation at 6000g at 4°C for 10 min. The supernatant was filter-sterilized with 0.2 mm-pore-size filter, and referred to as cell-free spent medium (CFSM). In case of the milk fermented by probiotics, CFSM was obtained by centrifugation of the fermented milk at 10,000g at 15°C for 15 min and then the supernatant was filter sterilized as mentioned above. The CFSM of all probiotic strains was stored at À20°C until use for further assays.
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