Author: Sun, Ying; Xiao, ShaoBo; Wang, Dang; Luo, Rui; Li, Bin; Chen, HuanChun; Fang, LiuRong
Title: Cellular membrane cholesterol is required for porcine reproductive and respiratory syndrome virus entry and release in MARC-145 cells Document date: 2011_12_16
ID: zrsnahi7_23
Snippet: To investigate the effect of MβCD on the cholesterol level of MARC-145 cells, MARC-145 cells were mock-treated or treated with various concentrations of MβCD. As shown in Figure 1A , MβCD treatment significantly reduced cellular cholesterol levels in a dose-dependent manner. When the concentration of MβCD was up to 10 mmol L ï€1 , cellular cholesterol level was reduced by approximately 75% compared with that in mock-treated cells. As the con.....
Document: To investigate the effect of MβCD on the cholesterol level of MARC-145 cells, MARC-145 cells were mock-treated or treated with various concentrations of MβCD. As shown in Figure 1A , MβCD treatment significantly reduced cellular cholesterol levels in a dose-dependent manner. When the concentration of MβCD was up to 10 mmol L ï€1 , cellular cholesterol level was reduced by approximately 75% compared with that in mock-treated cells. As the concentration of MβCD was further increased (>10 mmol L ï€1 ), cellular cholesterol level was no longer reduced, indicating that the remaining 25% cholesterol represented threshold levels of cholesterol which could not be extracted by higher doses of MβCD.
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