Selected article for: "cell suspension and virus production"

Author: Sun, Ying; Xiao, ShaoBo; Wang, Dang; Luo, Rui; Li, Bin; Chen, HuanChun; Fang, LiuRong
Title: Cellular membrane cholesterol is required for porcine reproductive and respiratory syndrome virus entry and release in MARC-145 cells
  • Document date: 2011_12_16
  • ID: zrsnahi7_27
    Snippet: To further elucidate the effects of MβCD on the replication cycle of PRRSV, mock-treated and MβCD-treated cells were infected with PRRSV strain CH-1a at 10 4.5 TCID 50 per well. Cell suspensions were harvested at various times post-infection (p.i.) and the titers of virus production in this cell suspension were determined. As shown in Figure 2B , virus reproduction was significantly inhibited in MβCDtreated cells, and virus titers at all stage.....
    Document: To further elucidate the effects of MβCD on the replication cycle of PRRSV, mock-treated and MβCD-treated cells were infected with PRRSV strain CH-1a at 10 4.5 TCID 50 per well. Cell suspensions were harvested at various times post-infection (p.i.) and the titers of virus production in this cell suspension were determined. As shown in Figure 2B , virus reproduction was significantly inhibited in MβCDtreated cells, and virus titers at all stages of the replication cycle were notably reduced compared with those in mock-treated cells. Specifically, at 36 h p.i., virus titers in MβCD-treated cells were 7.9×10 3 -fold lower than that in mock-treated cells. We also investigated the role of plasma membrane cholesterol in the infection of PRRSV strain WUH3, a highly pathogenic PRRSV that has been emerging in China in recent years. Significant inhibitory effects were observed ( Figure 2C) , indicating that the inhibitory effects of plasma membrane cholesterol on PRRSV replication are not strain-specific.

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