Title: Biosynthesis and processing of ribophorins in the endoplasmic reticulum Document date: 1984_9_1
ID: tuf1ih4g_5
Snippet: In this paper we demonstrate that the ribophorins themselves are products of bound polysomes which contain transient amino-terminal insertion signals and acquire asparaginelinked oligosaccharides during their co-translational insertion into ER membranes. Because all posttranslational changes that affect the ribophorins can be carried out by enzymatic activities found in the ER, the biosynthesis of these polypeptides does not require their transfe.....
Document: In this paper we demonstrate that the ribophorins themselves are products of bound polysomes which contain transient amino-terminal insertion signals and acquire asparaginelinked oligosaccharides during their co-translational insertion into ER membranes. Because all posttranslational changes that affect the ribophorins can be carried out by enzymatic activities found in the ER, the biosynthesis of these polypeptides does not require their transfer to the Golgi apparatus. Preparation of Antiserum: Antibodies against rat liver ribophorins ] and lI were produced and characterized as previously described (34) .
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