Author: Paquin, Ashley; Onabajo, Olusegun O.; Tang, Wei; Prokunina-Olsson, Ludmila
Title: Comparative Functional Analysis of 12 Mammalian IFN-?4 Orthologs Document date: 2016_1_1
ID: sqxrwgif_15
Snippet: Complete ORFs for IFN-l4 orthologs (Supplementary Table S1 ; Supplementary Data are available online at www.liebertpub.com/jir) were ordered as custom synthetic genes subcloned in pIDTBlue vector (IDT). The sequences were then PCR amplified and recloned into Halo-tag expression vector (Promega) to create constructs similar to the human IFN-l4-Halo plasmid. The final constructs were fully sequenced for validation, propagated in E. coli, and purifi.....
Document: Complete ORFs for IFN-l4 orthologs (Supplementary Table S1 ; Supplementary Data are available online at www.liebertpub.com/jir) were ordered as custom synthetic genes subcloned in pIDTBlue vector (IDT). The sequences were then PCR amplified and recloned into Halo-tag expression vector (Promega) to create constructs similar to the human IFN-l4-Halo plasmid. The final constructs were fully sequenced for validation, propagated in E. coli, and purified with an endotoxin-free Maxi plasmid kit (Qiagen).
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