Selected article for: "Luminex xTAG and previous study"
Author: Ko, Dae-Hyun; Kim, Hyun Soo; Hyun, Jungwon; Kim, Han-Sung; Kim, Jae-Seok; Park, Kyoung Un; Song, Wonkeun
Title: Comparison of the Luminex xTAG Respiratory Viral Panel Fast v2 Assay With Anyplex II RV16 Detection Kit and AdvanSure RV Real-Time RT-PCR Assay for the Detection of Respiratory Viruses
  • Document date: 2017_6_20
    • ID: vic6rbnb_25
    Snippet: For the specimens with discordant results, many showed weak positive reactions ("+" reactions, based on the cycles at which the melting curve analyses revealed the presence of the targets) in the initial Anyplex II RV16 assay. A previous study using serial dilutions of plasmid standards of several viruses showed that greater discrepancies existed in low-copy specimens [13] . However, the cycle threshold values for the AdvanSure RV assay and the f.....
    Document: For the specimens with discordant results, many showed weak positive reactions ("+" reactions, based on the cycles at which the melting curve analyses revealed the presence of the targets) in the initial Anyplex II RV16 assay. A previous study using serial dilutions of plasmid standards of several viruses showed that greater discrepancies existed in low-copy specimens [13] . However, the cycle threshold values for the AdvanSure RV assay and the fluorescence intensities for the Luminex xTAG RVP Fast v2 assays were highly variable; thus, the discrepant results may be related, at least in part, to the weak positive reactions, although other unexplained factors may also be involved. Future studies should investigate these possibilities.

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