Circulation of canine parvovirus among dogs living in human-wildlife interface in the Atlantic forest biome, Brazil

Vieira, Flávia V.; Hoffmann, Daniel J.; Fabri, Carolina U.F.; Bresciani, Katia D.S.; Gameiro, Roberto; Flores, Eduardo F.; Cardoso, Tereza C.

Sequences were aligned using BioEdit Sequence Alignment Editor V.7.0.9.0 (Tamura et al., 2007) . The evolutionary history was inferred using the Neighbour-Joining method (Saitou and Nei, 1987) . The percentages of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree (Hall, 1999) . All positions containing gaps and missing data were eliminated from the dataset. Phylogenetic analyses were conducted in MEGA4 (Tamura et al., 2007) . A phylogenetic tree was constructed based on amino acid alignments using BLAST analysis (www.blast.ncib.nlm.nih.gov/Blast.cgi), and sequences generated for CPV were submitted to GenBank and assigned.