Author: Mishra, Subodh Kumar; Shankar, Uma; Jain, Neha; Sikri, Kriti; Tyagi, Jaya Sivaswami; Sharma, Tarun Kumar; Mergny, Jean-Louis; Kumar, Amit
Title: Characterization of G-Quadruplex Motifs in espB, espK, and cyp51 Genes of Mycobacterium tuberculosis as Potential Drug Targets Document date: 2019_4_30
ID: qhkwn1on_8
Snippet: Further, we performed an electrophoretic gel mobility shift assay (EMSA) to check the presence of multimeric structures and determined the molecularity of these assemblies in solution. In the case of G4 folding, a fast-moving oligonucleotide band is considered to correspond to an intramolecular topology, whereas a slow-moving oligonucleotide band is likely to correspond to an intermolecular structure. [36] [37] [38] We examined the rate of migrat.....
Document: Further, we performed an electrophoretic gel mobility shift assay (EMSA) to check the presence of multimeric structures and determined the molecularity of these assemblies in solution. In the case of G4 folding, a fast-moving oligonucleotide band is considered to correspond to an intramolecular topology, whereas a slow-moving oligonucleotide band is likely to correspond to an intermolecular structure. [36] [37] [38] We examined the rate of migration for all MTB-PGQs oligos, as well as mutant sequences on native PAGE. All of the MTB-PGQ oligonucleotides migrate at a faster rate as compared with their mutant sequences, suggesting the formation of intramolecular complexes by all PGQs ( Figure S6 ).
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