Author: Harmand, Thibault J.; Pattabiraman, Vijaya R.; Bode, Jeffrey W.
Title: Chemical Synthesis of the Highly Hydrophobic Antiviral Membrane-Associated Protein IFITM3 and Modified Variants Document date: 2017_9_7
ID: svivcw92_41
Snippet: Fluorescent-S1-ArgKA S2 was synthesized by loading the cyanosulfurylide linker on Rink amide polystyrene resin (loading 0.36 mmol/g, scale: 0.15 g, 0.05 mmol) using the protocol previously reported by our group. 3 Fmoc-Arg(Pbf)-OH was coupled to the cyanosulfurylide using HATU and NMM in DMF for 4 h followed by several washings with DMF, sodium diethyldithiocarbamate, DMF and CH 2 Cl 2 . After drying the loading was determined to be 0.3 mmol/g......
Document: Fluorescent-S1-ArgKA S2 was synthesized by loading the cyanosulfurylide linker on Rink amide polystyrene resin (loading 0.36 mmol/g, scale: 0.15 g, 0.05 mmol) using the protocol previously reported by our group. 3 Fmoc-Arg(Pbf)-OH was coupled to the cyanosulfurylide using HATU and NMM in DMF for 4 h followed by several washings with DMF, sodium diethyldithiocarbamate, DMF and CH 2 Cl 2 . After drying the loading was determined to be 0.3 mmol/g.
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