Author: Al-Mulla, Hawaa M. N.; Turrell, Lauren; Smith, Nicola M.; Payne, Luke; Baliji, Surendranath; Züst, Roland; Thiel, Volker; Baker, Susan C.; Siddell, Stuart G.; Neuman, Benjamin W.
Title: Competitive Fitness in Coronaviruses Is Not Correlated with Size or Number of Double-Membrane Vesicles under Reduced-Temperature Growth Conditions Document date: 2014_4_1
ID: tfuupgkg_20
Snippet: In order to further test whether the differential plaque assay results reflected the proportion of wild-type and ts RNA released into the supernatant at the end of a competition assay in 17Cl-1 cells, RT-PCR and DNA sequencing (39) were performed for competition assays containing Brts105 and wild-type virus ( Fig. 5C and D) . In this assay, three replicates gave a fitness of 0.8 Ï® 0.1 for Brts105, which fell within the range of results recorded.....
Document: In order to further test whether the differential plaque assay results reflected the proportion of wild-type and ts RNA released into the supernatant at the end of a competition assay in 17Cl-1 cells, RT-PCR and DNA sequencing (39) were performed for competition assays containing Brts105 and wild-type virus ( Fig. 5C and D) . In this assay, three replicates gave a fitness of 0.8 Ï® 0.1 for Brts105, which fell within the range of results recorded by plaque assay (0.4 to 6.4). The parity between Brts105 and the wild type was maintained even when the relative abundance of each virus at the beginning of the competition was altered (Fig. 5D ). This confirmed that Brts105 was not significantly less fit than wildtype virus under these test conditions.
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