Author: Harmand, Thibault J.; Pattabiraman, Vijaya R.; Bode, Jeffrey W.
Title: Chemical Synthesis of the Highly Hydrophobic Antiviral Membrane-Associated Protein IFITM3 and Modified Variants Document date: 2017_9_7
ID: so0685rt_43
Snippet: The crude peptide (100 mg) was dissolved in a glass vial with 4.0 mL of H 2 O/CH 3 CN (1:1) and 0.1% TFA. After complete dissolution, Oxone (541 mg, 0.88 mmols, 50 equiv) was added. The reaction mixture was placed on a shaker and gently agitated for 5 minutes. The reaction was quenched with 250 µL of DMS. The excess of DMS was evaporated using a N 2 flow and the resulting mixture was re-dissolved, centrifuged and purified by preparative HPLC usi.....
Document: The crude peptide (100 mg) was dissolved in a glass vial with 4.0 mL of H 2 O/CH 3 CN (1:1) and 0.1% TFA. After complete dissolution, Oxone (541 mg, 0.88 mmols, 50 equiv) was added. The reaction mixture was placed on a shaker and gently agitated for 5 minutes. The reaction was quenched with 250 µL of DMS. The excess of DMS was evaporated using a N 2 flow and the resulting mixture was re-dissolved, centrifuged and purified by preparative HPLC using Shiseido Capcell pak C18 column (20 x 250 mm) with a gradient of 10 to 65% CH 3 CN (with 0.1% TFA) in 30 min to obtain 13 mg of Fluorescent-S1-ArgKA S2 in an overall yield of 14%.
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