Author: Feng, Mingqian; Bian, Hejiao; Wu, Xiaolin; Fu, Tianyun; Fu, Ying; Hong, Jessica; Fleming, Bryan D; Flajnik, Martin F; Ho, Mitchell
Title: Construction and next-generation sequencing analysis of a large phage-displayed V(NAR) single-domain antibody library from six naïve nurse sharks Document date: 2018_11_7
ID: wc6k06sm_19
Snippet: We hypothesize that the diversity of this large naïve shark library can be further increased significantly by randomizing the CDR1 sequences and keeping the highly diverse CDR3 [33, 34] . For example, the eight amino acids in CDR1 can be completely randomized to further diversify our V NAR library. Furthermore, affinity maturation of the binders in Figure 6 can be performed by randomizing CDR1. In summary, we believe this shark library has a sui.....
Document: We hypothesize that the diversity of this large naïve shark library can be further increased significantly by randomizing the CDR1 sequences and keeping the highly diverse CDR3 [33, 34] . For example, the eight amino acids in CDR1 can be completely randomized to further diversify our V NAR library. Furthermore, affinity maturation of the binders in Figure 6 can be performed by randomizing CDR1. In summary, we believe this shark library has a suitable size and diversity for antibody discovery and can potentially be used as a large single-domain antibody discovery platform.
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