Author: Feng, Mingqian; Bian, Hejiao; Wu, Xiaolin; Fu, Tianyun; Fu, Ying; Hong, Jessica; Fleming, Bryan D; Flajnik, Martin F; Ho, Mitchell
Title: Construction and next-generation sequencing analysis of a large phage-displayed V(NAR) single-domain antibody library from six naïve nurse sharks Document date: 2018_11_7
ID: wc6k06sm_23
Snippet: The GPC3-positive human hepatocellular carcinoma cell line HepG2 was maintained as adherent monolayer cultures in DMEM medium (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (HyClone, Logan, UT), 1% L-glutamine, and 1% penicillin-streptomycin (Invitrogen) in a 5% CO 2 incubator at 37 • C. GPC3-negative A431 cells (Human epithelial carcinoma cell line) were engineered in our laboratory to express high levels of human GPC3 by .....
Document: The GPC3-positive human hepatocellular carcinoma cell line HepG2 was maintained as adherent monolayer cultures in DMEM medium (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (HyClone, Logan, UT), 1% L-glutamine, and 1% penicillin-streptomycin (Invitrogen) in a 5% CO 2 incubator at 37 • C. GPC3-negative A431 cells (Human epithelial carcinoma cell line) were engineered in our laboratory to express high levels of human GPC3 by transfection with a plasmid encoding full-length GPC3 [38, 39] . Both A431 and the stably transfected cells (G1) [39] were maintained in DMEM with supplements.
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